NBC News, Dmitri S. Peskov, the Kremlin’s spokesman, said that the two Americans, Alex Drueke, 39, and Andy Tai Ngoc Huynh, 27, were “soldiers of fortune” who had been engaged in shelling and firing on Russian forces and should be “held responsible for the crimes they have committed.”

The sanctions imposed on Russia also played a role on Monday in an escalating confrontation with Lithuania, a member of both the European Union and NATO.

The Russian authorities threatened Lithuania with retaliation if the Baltic country did not swiftly reverse its ban on the transportation of some goods to Kaliningrad, the Russian exclave between Lithuania and Poland. Citing instructions from the European Union, Lithuania’s railway on Friday said it was halting the movement of goods from Russia that have been sanctioned by the European bloc.

Mr. Peskov told reporters the situation was “more than serious.” He called the new restrictions “an element of a blockade” of the region and a “violation of everything.”

small town of Toshkivka in Luhansk Province, part of the eastern region known as Donbas. That is where Russian forces have concentrated much of their military power as part of a plan to seize the region after having failed to occupy other parts of the country, including Kyiv, the capital, and Kharkiv, the second-largest city, in northern Ukraine.

Reports over the weekend suggested that Russian forces had broken through the Ukrainian front line in Toshkivka, about 12 miles southeast of the metropolitan area of Sievierodonetsk and Lysychansk. Those are the last major cities in Luhansk not to have fallen into Russian hands. As of Monday, it remained unclear whether Russia had made any further advance there.

But Ukrainian officials said Russian forces had intensified shelling in and around Kharkiv, weeks after the Ukrainians had pushed them back, suggesting that Moscow still had territorial ambitions beyond Donbas.

“We de-occupied this region,” Mr. Zelensky said in an address to a conference of international policy experts in Italy. “And they want to do it again.”

Matthew Mpoke Bigg reported from London, Andrew Higgins from Warsaw, Thomas Gibbons-Neff from Druzhkivka, Ukraine, and Rick Gladstone from New York. Reporting was contributed by Valerie Hopkins and Oleksandr Chubko from Kyiv; Dan Bilefsky from Montreal; Monika Pronczuk from Brussels; Austin Ramzy from Hong Kong; Stanley Reed from London; and Zach Montague from Rehoboth Beach, Del.

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